Custom Oligonucleotide Service:
Genei, continues to offer custom oligonucleotide service to scientists nationwide. We
are happy to announce the new service
Genei, continues to offer custom oligonucleotide service to scientists nationwide. We
are happy to announce the new service desalted oligo
(1-2 ODs) for a very competitive
price of Rs. 50 per base.
Features:
1. |
Oligonucleotides available
as (a) Crude (b) Desalted (c) PAGE purified. |
2. |
Choice
of purification (a) Desalting: using sephadex G-25 columns (b) |
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PAGE purification: 20%
polyacrylamide + 7M urea gel electrophoresis. |
3. |
Modifications
available (a) Inosine - at any position other than 3' end (b) |
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Biotin and Fluorescein -
only at 5' end. |
4. |
Certificate of analysis
includes primer length, sequence, yield, |
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concentration and purity. |
5. |
Product quality checked by
PAGE analysis of the primer. |
6. |
Oligos will be supplied in
lyophilised form. |
7. |
Quick delivery. |
8. |
On line ordering -
genei@sanmargroup.com |
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Note:
- Order should accompany primer sequence
with indication of 5' - 3'.
- No mixed bases at 3' end.
- Please use the IUPAC-code for mixed bases
(M=A/C, R=A/G, W=A/T, Y=C/T, S=C/G, K=G/T, H=A/C/T, V=A/C/G, D=A/G/T, B=C/G/T, N=A/C/G/T).
Crude oligo: Deprotected oligo with 85-90% of full length
product along with 10-15% of failure sequences, salts and protective chemical groups.
Application: Standard PCR, Hybridization, Probing etc.
Desalted oligo: Desalting of
crude oligo on Sephadex G-25 column, removes ammonium salts and protective chemical
groups.
Application: PCR, Probing,
Cycle sequencing, Mobility shift, Hybridization, Primer extension.
PAGE Purified oligo: Crude oligo purified
on 20% Urea PAGE followed by desalting on Sephadex G-25 yields 95-99% purity. Total yield
is lower due to relative ineffective recovery of oligo from the gel and Sephadex G-25
column. The basis of separation of the oligo is by molecular weight and charge.
Application: Cloning, RT-PCR, Site Directed Mutagenesis, Gel Shift Assay, DNA
fingerprinting, in situ hybridization, Microsatellite polymorphism, Gene synthesis.
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