Site directed mutagenesis is an important tool for
structure-function analysis of proteins. Recent developments in molecular biology and
molecular biophysics utilise techniques for introducing mutations in putative activation
domains in proteins. Enhancement or down regulation of biological activities following
mutagenesis studies provide important details about the structure and function of the
protein. Genei proudly introduces the unique facility of introducing mutations ( single to
multiple bases ) in your protein gene.
Salient Features:
1)
Mutation by base
substitution at the desired position.
2)
Single base substitution to
multiple base substitutions ( upto 5
substitutions within 15 bp ) can be performed.
3)
The
steps in the mutagenesis services could typically include :
(a)
Sub-cloning of DNA
fragment into a mutagenesis vector.
(b) Designing & synthesising of mutation primer.
(c) Incorporation of mutation by PCR
(d) Confirmation of mutation by sequencing
(e)
Cloning of mutated fragment back to original vector.
Each order should accompany the following
information:
Details of the Clone :
A)
Vector
:
Name, size, site at which cloned.
Insert
:
Size, sequence whether G/C rich, A/T rich, has a
secondary structure, long repeats, homopolymer regions - others.
B)
Sample sent as
:
Purified DNA / stab / plate
C)
Growing condition :
Host, antibiotic used and temperature
Service Report:
The report will include sequence data and mutated cloned DNA (5
µgs, purified, lyophilised DNA).
How to send samples:
DNA must be transported in dry ice /
coolant packs at sufficiently low temperature to prevent degradation. DNA in solution
should not be sent at room temperature. DNA can be sent as lyophilised sample. Care should
be taken to see that lyophilised DNA is not excessively dry as this leads to problem in
reconstitution.
Delivery time:
SER 26 :
4-6 weeks. SER 27 : 6-8 weeks. Shipping Address:
The mutagenesis services include incorporation of
single mutation or multiple mutations (dispersed or within a short stretch of DNA) into a
cloned DNA fragment. The process invloves subcloning of DNA fragment into mutagenesis
vector, designing of mutation primer, incorporation of mutation,
confirmation of mutation by sequencing and cloning of mutated fragment back to original
vector. The report will include sequence data and mutated cloned DNA (5 µgs, purified,
lyophilised DNA).
SER26
Mutagenesis
Service 1
(Single
to multiple mutations within 15 bp stretch in clones smaller
than 5.0 kb)
Per sample
SER27
Mutagenesis
Service 2
(Single
to multiple mutations within 15 bp stretch in clones smaller
than 5.0 kb)
