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Ultrapure
Prep Kits
Ultrapure
prep kits provide a simple and convenient method of
purification of plasmid DNA and genomic DNA. The procedure
is based on the selective binding of nucleic acids from
crude extract to an anion exchange resin. DNA binds to the
resin in a gravity flow column under appropriate salt
concentration and pH while contaminants such as proteins,
polysaccharides flow through. Impurities are further
removed by a medium salt buffer wash. Finally DNA is
eluted in a high salt buffer, desalted and concentrated by
alcohol precipitation, reconstituted in Tris buffer or
water.
Highlights:
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Purity
of DNA comparable to that obtained by Cesium
chloride gradient centrifugation. |
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Reproducible
yields of ultrapure DNA. |
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No RNA
contamination. |
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No
phenol, chloroform handling. |
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Purified
DNA delivers optimal results in any down stream
applications. |
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The
A260/A280 ratio of DNA lies between 1.6 to 2.0. |
Geneispin
Prep Kits
Geneispin
prep kits provide a rapid and reliable method of
purification of plasmid and high molecular weight genomic
DNA. Geneispin kits use silica gel/membrane in a
convenient spin column format. Samples are lysed using
optimal buffers mixed with alcohol and applied to the
column. The nucleic acid specifically adsorb to the
membrane or matrix under high salt conditions while
contaminants flow through. Inhibitors are completely
removed by an effective wash step and the pure nucleic
acid is eluted in minimum volume of Tris buffer or water.
Highlights:
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Rapid,
efficient and economical. |
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No
phenol/chloroform extraction. |
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No
alcohol precipitation. |
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Ready
to use DNA. |
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The
A260/A280 ratio of DNA lies between 1.6 to 2.0. |
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DNA
Extraction Kits/Solution
DNA
Extraction kits/solution contain complete, ready to use
reagents for the isolation of genomic DNA from animal and
plant origin. It is based on the use of the cell lysis
solution that hydrolyses RNA, denatures the proteins and
other contaminants allowing selective precipitation of DNA
from the cell lysate. The isolated DNA can be used for
many molecular biology applications.
Highlights:
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Quick,
single tube method for DNA extraction. |
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Reliable
and reproducible yields of genomic DNA. |
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Isolated
DNA suitable for restriction digestion and PCR
amplification. |
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Large
number of samples can be handled. |
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No
phenol-chloroform extraction. |
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